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WP 2.2 Functional Validation

Task 2.2-1 shRNA mediated gene knockdown in the rat.
Task 2.2-2 Gene targeting in rat ES cells by homologous recombination.
Task 2.2-3 Transposon-mediated rat genome manipulation.
Task 2.2-4 Gene ablation using ZFNs in the rat.
Task 2.2-5 Sperm archive for ENU mutants.
Task 2.2-6 Isolation of single gene polymorphic/mutated congenic rats.
Task 2.2-7 Phenotypic validation of rat models.

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